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The laboratory of ďStem Cells and NeurogenesisĒ, Division of Neuroscience, San Raffaele Scientific Institute is seeking for scientific personnel to expand current projects on Parkinsonís disease and neuronal reprogramming. The lab has established new procedures to convert skin fibroblasts into functional neurons by the expression of a set of reprogramming transcription factors (Caiazzo et al., Nature 476:224-7, 2011; DellíAnno et al. JCI, 2014 In press). We have conceived a new approach to delay and arrest Parkinsonís pathology and wish to investigate a specific molecular mechanism contributing to disease progression. In addition, we wish to strength our direct neuronal reprogramming approach starting from adult human somatic cells (fibroblasts and blood cells) and explore their therapeutic potential in an animal model of Parkinsonís disease. The laboratory is strategically located within the scientific environment of the San Raffaele Biomedical Area.

 

 

Research fellow

Project: Understanding and blocking the spreading mechanisms contributing to Parkinsonís disease progression. This project aims to understand the cellular bases by which Parkinsonís disease pathology is spreading over the brain during its clinical progression. The approach will entail the use of alpha-synuclein transgenic mice already available in the lab. In addition, a new molecular process associated to microglia dysfunction will be studied to assess its contribution to the origin and progression of the disease.

 

 

Research fellow

Project: Generating human iDA neurons and testing their therapeutic potential. This person will be responsible to establish new procedures of direct neuronal reprogramming starting from post-natal and adult human fibroblasts. Human reprogrammed dopaminergic (DA) neurons will be then transplanted in Parkinsonís disease animal model and tested for acquired neuronal and synaptic activities. Genome-wide expression profiling will be carried out comparing iPS- and fibroblast-derived DA neurons. This genomic analysis is meant to identify new and critical fate determining factors of the dopaminergic neuronal commitment and differentiation. Applicants are expected to be highly motivated and creative, have good organization skills and able to interact and collaborate within a large research group.

 

 

Research fellow

Project: As part of the focus of the Neuromedicine research group at the CBM, we are investigating the molecular and neurobiological bases of Parkinsonís disease (PD). This post will be for a project jointly coordinated between the Neuromedicine group at the CBM in Bolzano and the San Raffaele Scientific Institute (ďStem Cells and NeurogenesisĒ group headed by Dr. Vania Broccoli) in Milan. The successful candidate will master human iPS cell cultures and their differentiation into functional neurons. Furthermore, she/he will establish the CRISPR/Cas9 gene editing technology for site-specific engineering of the cell lines. In particular, the CRISPR/Cas9 nuclease system will be used for gene correction in PD patient-specific iPS cell lines to generate control isogenic cells. In addition, PD related genes will be fused with appropriate tags with the aim of isolating interactors and to determine the spatial dynamics of their respective protein products. Read more

 

 

Undergraduate internship

Students are welcome to apply to attend the lab for their diploma thesis.

 

 

Interested candidates should send a detailed curriculum vitae by e-mail to: broccoli.vania@hsr.it.